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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Alcohol Clin Exp Res. 2011 Mar 29;35(8):1550–1556. doi: 10.1111/j.1530-0277.2011.01492.x

Figure 2. Alcohol Induces HDAC2 Gene and this Effect is Inhibited by TSA.

Figure 2

After reaching confluency, SK-N-MC were pre-treated with TSA (50 nM) for 2 hours, then treated with EtOH (0.1%) for 24 hours. After incubation, cells were harvested, RNA was extracted and reverse transcribed followed by qRT- PCR for HDAC2 and housekeeping GAPDH and 18sRNA gene expression. EtOH significantly enhanced HDAC2, while TSA + EtOH treatment significantly inhibited HDAC2 gene. Data are expressed as mean ± SEM of TAI values of at least three independent experiments. # represents significance compared to control. * represents significance compared to EtOH treatment.