Figure 4. Alcohol Induces ROS Production and this Effect is Inhibited by Antioxidants and TSA.

SK-N-MC (1 × 10⁵ cells) were incubated in 96-well plates overnight. Then, the cells were pre-incubated with TSA or antioxidants: uric acid (50 µM) or catalase (0.001 mg) for 2 hours, DCF-DA (100 µM) for 1 hour, then treated with EtOH or H₂O₂ (50 µM). Levels of ROS were measured with the Biotek Synergy HT plate reader. The fluorescence was detected at 485 excitation and at 528 emission spectra. Data are expressed as mean ± SE of RFU values of six independent experiments. A value of p ≤ 0.05 was indicative of significance. # represents significance compared to control. * represents significance compared to EtOH treatment.