Table 2.
Strains used in present study
| Strain
|
Genotype
|
|---|---|
| JY112a | α his3-Δ200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-Δ63 |
| JY113a | a his3-200 leu2Δ1∷LEU2-Deg1-lacZ ura3-52 lys2∷LYS2-Deg1-URA3 trp1-Δ63 |
| MHY496b | MHY501 with ubc6-Δ1∷HIS3 |
| MHY498b | MHY501 with ubc4-Δ1∷HIS3 |
| MHY500b | a his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 |
| MHY501b | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 |
| MHY503b | MHY501 with ubc4-Δ1∷HIS3 ubc6-Δ1Δ∷HIS3 |
| MHY513b | MHY500 with ubc4-Δ1∷HIS3 |
| MHY551b | MHY500 with ubc7∷LEU2 |
| MHY552b | MHY501 with ubc6-Δ1∷HIS3 ubc7∷LEU2 |
| MHY884c | MHY500 with doa4Δ∷leu2∷HIS3 |
| MHY1364d | MHY501 with cue1Δ∷HIS3 |
| MHY1366 | MHY500 with prc1-1 |
| MHY1367 | MHY500 with cue1Δ∷HIS3 ubc6Δ∷TRP1 ubc7∷LEU2 prc1-1 |
| MHY1410 | α his3-200∷pRS303(HIS3) leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-Δ63 |
| MHY1411 | a his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-Δ63∷pRS304(TRP1) |
| MHY1412 | α his3-Δ200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1 ubc7∷LEU2 |
| MHY1631 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 doa10Δ∷HIS3 |
| MHY1648 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 doa10Δ∷HIS3 ubc4∷HIS3 |
| MHY1651 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 doa10Δ∷HIS3 prc1-1 |
| MHY1657 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 DOA10-myc9∷his5+ |
| MHY1658 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 DOA10-GFP∷HIS3 |
| MHY1669e | MHY501 with hrd1Δ∷LEU2 |
| MHY1670 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 doa10Δ∷HIS3 ubc6Δ∷HIS3 |
| MHY1690 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 ubc7∷LEU2 DOA10-myc9∷his5+ |
| MHY1703 | MHY501 with hrd1Δ∷LEU2 doa10Δ∷HIS3 |
| MHY1740 | α his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2∷LYS2-Deg1-URA3 trp1-Δ63 doa11-1 doa12-1 |
| MHY1745 | α his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2∷LYS2-Deg1-URA3 trp1-Δ63 doa11-1 |
| MHY1747 | a his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-Δ63∷pRS304(TRP1) doa12-1 |
| MHY2091 | α his3-200 leu2-3,112 ura3-52 lys2-801 trp1-1 doa10-C39S-myc9∷his5+ |
| MHY2093f | a leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801 rpn4∷URA3(mTn3) |
| MHY2094 | α his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-1 doa10-2 |
| MHY2095 | α his3-200 leu2-Δ1∷LEU2-Deg1-lacZ ura3-52 lys2-801∷LYS2-Deg1-URA3 trp1-1 doa10-3 |
a
J. Laney and M. Hochstrasser, unpubl.
c
M. Hochstrasser, unpubl.