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. 2001 Oct 15;15(20):2702–2719. doi: 10.1101/gad.915701

Figure 2.

Figure 2

Up-regulation of GATA-4 activity induces contractile protein gene expression and sarcomere reorganization in cardiomyocytes. (a) Western blot analysis of nuclear extracts from cardiomyocytes and 293 cells infected with a lacZ control or with GATA-4-expressing adenovirus. (b) Increased GATA DNA-binding activity in cardiomyocytes infected with the GATA-4 adenovirus. EMSAs were performed using nuclear extracts from cardiomyocytes infected with lacZ or GATA-4 adenovirus and the ANF −120-bp GATA probe. (c) Overexpression of GATA-4 induces cardiac gene expression. Total RNA (20 μg) extracted from cardiomyocytes infected with lacZ or GATA-4 adenovirus was analyzed by Northern blot and quantified by PhosphorImager, as described in Materials and Methods. (d) Up-regulation of GATA-4 activity induces cardiomyocyte sarcomere reorganization. Cardiomyocytes infected with lacZ or GATA-4 adenovirus were fixed and costained using phalloidin-FITC (green) and anti-GATA-4 antibody (red). (e) Quantification of the percentage of reorganized cardiomyocytes. Cells were scored as described in Material and Methods.