Figure 3.

Lif2p is down-regulated in MATa/MATα cells. Haploid MATa strains Lev412 (Lif2p_myc), Lev413 (Dnl4p_myc), Lev368 (Lif1p_myc), Lev359 (Yku70p_myc), and Lev355 (Yku80p_myc) were transformed with an integrative plasmid bearing MATα (sp427) or crossed with MATα strain Lev360. Total proteins were extracted with TCA and 30 μg were analyzed by Western blotting using monoclonal 9E10 anti-myc antibody. All tagged alleles behaved as wild type when tested for NHEJ (data not shown). (h) Haploid; (d) diploid.