Table 1.
Survival frequency in strains with a continuously induced DSB
| Relevant genotype
|
Survival (×106)a with HO
|
Survival (×106)a with I-SceI
|
|---|---|---|
| Haploid MATa strains | ||
| 1 wild type | 970 ± 140 | 1700 ± 610 |
| 2 dn14Δ | 10 ± 7 | 14 ± 8 |
| 3 lif1Δ | 8 ± 6 | n.d. |
| 4 lif2Δ | 6 ± 3 | 7 ± 5 |
| 5 lif1Δ lif2Δ | 14 ± 9 | n.d. |
| 6 lif2Δ pCEN-LIF2 | 960 ± 150 | 2600 ± 680 |
| 7 wild-type p2μ-LIF2 | 990 ± 160 | 2600 ± 400 |
| Haploid MATa/MATα strains | ||
| 8 wild type | n.d. | 30 ± 20 |
| 9 wild type p2μ-LIF2 | n.d. | 190 ± 70 |
| 10 dnl4Δ | n.d. | 11 ± 3 |
| 11 dnl4Δ p2μ-LIF2 | n.d. | 38 ± 26 |
| Diploid MATa/MATα strains | ||
| 12 wild type | 150 ± 50 | n.d. |
| 13 wild type p2μ-LIF2 | 870 ± 140 | n.d. |
| 14 dnl4Δ/dnl4Δ | <1 | n.d. |
| 15 dnl4Δ/dnl4Δ p2μ-LIF2 | <2 | n.d. |
All haploid strains expressing HO were isogenic to Lev 348. Diploid strains were obtained by crossing Lev 348 × Lev 360 (wild type) and Lev 349 × Lev 363 (dn14Δ/dn14Δ). All strains expressing I-SceI were isogenic to Lev 338. (n.d.) Not determined.
a
The mean and standard deviation were calculated by averaging three samples.