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. 2011 Apr 21;12(6):558–564. doi: 10.1038/embor.2011.52

Figure 4.

Figure 4

LGR4-deficient phenotype ex vivo is partly rescued by LiCl. (A) Crypts from P15 Lgr4 knockout cultured in the presence or absence of 7.5-mM LiCl. One representative experiment out of 11 is shown. (B) Dependence of LGR4-deficient organoids on LiCl treatment. After dissociation, Lgr4-knockout-LiCl rescued organoids were replated in the presence (+LiCl) or absence (−LiCl) of LiCl. Scale bars, 50 μm. (C) Quantitative real-time–PCR analysis at day 1 of transcripts from P15-derived Lgr4-knockout crypts cultured with or without 5-mM LiCl. Transcripts were normalized to untreated samples. Statistical analyses: values are means±s.e.m. Significance was computed from paired t-test: *P<0.03, **P=0.0024, ***P⩽0.0005. (D) Ontogeny analysis of microarray results with the David software, comparing gene expression at day 1 of Lgr4 knockout with wild-type crypts cultured in control medium (−LiCl), and knockout crypts treated with or without LiCl.