Figure 2.
ceRACK-1 affects the level of miRNA in an alg-1-dependent manner. (A) Although alg-1(RNAi) reduces miRNA levels, rack-1(RNAi) leads to an accumulation of miRNAs. (B) ALG-1, but not ALG-2, is important to accumulate miRNAs in rack-1(RNAi) animals. The miRNA levels were measured relative to the small nucleolar RNA (sn2841) using quantitative reverse transcription–PCR (TaqMan Assays) in adult animals fed with bacteria expressing either control (Ctl), alg-1 or rack-1 double-stranded RNA, as indicated. In all RNAi conditions, no significant changes were observed in the level of the control RNA sn2841 (data not shown). The error bars represent the 95% confidence interval from three independent experiments and a Student's two-sided t-test was applied on the normalized Ct values to obtain P-values. miRNA, microRNA; RACK, receptor for activated C-kinase; RNAi, RNA interference; Wt, wild type.