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. 2011 May 13;12(6):594–601. doi: 10.1038/embor.2011.77

Figure 3.

Figure 3

Diadenylate cyclase activity is required for DisA foci assembly. (A,B) MB3 (disA-gfp) and YA127 (disAD77N-gfp) cells were induced to sporulate and subjected to fluorescence microscopy. FM4-64 (red) and signal from GFP (green) are shown. (A) MB3 (upper panels) and YA127 (lower panels) cells at 1.5 h of sporulation. (B) YA127 cells at 2.5 h of sporulation in the absence (upper panels) and presence of 350 μg/ml NAL (lower panels), added at 0 h of sporulation. (C) Percentage of sporulating MB3 (disA-gfp), YA5 (ΔdisA) and Y127 (disAD77N-gfp) cells at 3.5 h of sporulation in the absence (–) or presence (+) of 350 μg/ml NAL, added at 0 h of sporulation. Cells were visualized using fluorescence microscopy, with the appearance of polar septa serving as the marker of sporulation. One representative experiment out of three independent experiments is shown. Standard deviation was calculated from the means of several fields of cells. At least 600 cells were counted for each treatment. GFP, green fluorescent protein; NAL, nalidixic acid.