FIG. 3.
No additive effect when the AM stroma and HOXB4 induction strategies are combined. Cos7 cells, transiently transfected with the pCAGHOXB4ERT2IP construct, cultured in the absence (A) or the presence (B) of tamoxifen followed by immunocytochemical analysis using an anti-HOXB4 antibody (green) and counterstaining of the nucleus with DAPI (blue). (C) Number of hematopoietic colonies observed when control CGR8 embryoid bodies (EBs) and CGR8-HOXB4-ERT2 inducible EBs were cultured in the presence (+) or absence (−) of tamoxifen (added on Days 1, 3, and 5 of differentiation) and/or co-cultured with AM14.1C4 or OP9 stromal cell layers. CFU assays were setup at 6 days differentiation.