Table 2.
Analysis of Single EBs Differentiated in Co-Culture
| Co-culture of single EBs | % EBs with hematopoietic activity | Hematopoietic colonies per EB | |
|---|---|---|---|
| 6 days differentiation | |||
| Gelatin | Contact (n = 207) | 52.7 | 3.2 ± 6.9 |
| Noncontact (n = 24) | 37.5 | 4.5 ± 8.9 | |
| AM20.1B4 | Contact (n = 201) | 64.2* | 16.8 ± 33.7* |
| Noncontact (n = 24) | 16.7† | 0.6 ± 2.2† | |
| UG26.1B6 | Contact (n = 47) | 42.6 | 1.7 ± 3.7 |
| Noncontact (n = 24) | 20.8 | 0.4 ± 1.1 | |
| EL08.1D2 | Contact (n = 48) | 27.1* | 0.4 ± 0.7* |
| Noncontact (n = 24) | 29.2 | 0.5 ± 0.9 | |
| 10 days differentiation | |||
| Gelatin | Contact (n = 48) | 68.3 | 11.1 ± 12.2 |
| Noncontact (n = 24) | 70.8 | 10.3 ± 13.4 | |
| AM20.1B4 | Contact (n = 48) | 93.5* | 37 ± 27.9* |
| Noncontact (n = 24) | 47.8† | 11 ± 14.3† | |
| UG26.1B6 | Contact (n = 48) | 60.9 | 7.0 ± 10.6 |
| Noncontact (n = 24) | 54.2 | 8.8 ± 10.3 | |
| EL08.1D2 | Contact (n = 207) | 52.7 | 3.2 ± 6.9 |
| Noncontact (n = 24) | 37.5 | 4.5 ± 8.9 | |
The proportion of individual EBs with associated hematopoietic activity and the number of hematopoietic colonies detected within individual dissociated EBs after culture on gelatin or in direct contact with stromal cells. In noncontact conditions, contact between EBs and stromal cells was prevented by the presence of transwell inserts. The number (n) of EBs (7a-GFP) analyzed in each experiment is shown.
*
P < 0.004 comparison of contact cultures: stromal co-culture versus corresponding gelatin culture.
†
P < 0.01 comparison of noncontact versus corresponding contact culture.