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. 2001 Nov 15;15(22):2980–2990. doi: 10.1101/gad.205401

Figure 1.

Figure 1

Molecular cloning of FHY1. (A) Genetic and physical map of part of chromosome 2 containing FHY1. FHY1 was mapped using 714 (rec. 1) and 1708 (rec. 2) chromosomes that were recombinant between FHY1 and the markers cop1-6 and nga168, respectively. We positioned FHY1 on a contig of YACs and BACs (see Materials and Methods) and identified a mutation in the sequence of ORF 18 in the fhy1-1 mutant. The structure of FHY1 is shown (thin line, introns; thick line, ORF; filled thick line, noncoding 5′ and 3′ transcribed sequence) with the position of the fhy1-1 mutation. FHY1 corresponds roughly to, but differs substantially from, the last part of the ORF 18 originally identified by the genefinder program. (B) Genomic DNA gel-blot analysis showing the rearrangement in fhy1-2. DNA was digested with BglII and probed with a radiolabeled FHY1 probe. (C) An FHY1 probe reveals FHY1 transcripts in FHY1, fhy1-1 but not fhy1-2 plants. (Upper panel) RNA gel-blot hybridization of RNA from wild type (WT), fhy1-1, and fhy1-2. (Lower panel) UV fluorescence of RNA blotted and hybridized in panel above. (D) A genomic DNA fragment containing FHY1 complements the fhy1-2 phenotype. Seedlings grown 5 d in cFR are shown; 343 is an fhy1-2 homozygote that is also homozygous for an FHY1-containing transgene.