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Extracellular matrix proteins on day 30 of differentiation. (A) Embryonic stem cell (ESC) aggregates from day 30 of bioreactor differentiation. (B) Aggregates expressed Alcian blue and Alizarin red. (C) Calcium content, alkaline phosphatase activity, and proteoglycan content in each bioreactor-differentiated culture are shown. An analysis of variance (ANOVA) was performed at each time point (*P < 0.01, **P < 0.001).
