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Lineage-specific protein marker expression. (A) Representative images depict the expression of 4 markers (collagen II, osteocalcin, aggrecan, collagen X) on day 30 of bioreactor differentiation. (B) The degree of expression of each marker was graded from 0 to 2 (0, no positive cells; 1, few positive cells; 2, majority of cells in the aggregates were positive; dash (-), sample not assessed).
