Table 1.
Buffering of Internal Free Mg2+ and Mg2+-ATP Concentrations
| Internal solution | ||
| Standard intracellular pipette filling solution (SIS) contained (in mM) | ||
| Cs-Glutamate 140, NaCl 8, Cs-BAPTA 10, HEPES-CsOH 10, pH 7.2 adjusted with CsOH | ||
| For Figures 3 and 4 (HEKs and MSCs) | ||
| 0 mM free Mg2+ | SIS | |
| 30 μM free Mg2+ | SIS + 39 μM MgCl2 | |
| 120 μM free Mg2+ | SIS + 155 μM MgCl2 | |
| 211 μM free Mg2+ | SIS + 271 μM MgCl2 | |
| 788 μM free Mg2+ | SIS + 1.008 mM MgCl2 | |
| 1,595 μM free Mg2+ | SIS + 2.030 mM MgCl2 | |
| For Figure 5 (MSCs—always 211 μM free Mg2+) | ||
| 0 mM Mg2+-ATP | SIS + 271 μM MgCl2 | (0 mM free ATP) |
| 1 mM Mg2+-ATP | SIS + 1.27 mM MgCl2 + 1.5 mM Na+-ATP | (0.5 mM free ATP) |
| 2 mM Mg2+-ATP | SIS + 2.27 mM MgCl2 + 3 mM Na+-ATP | (1 mM free ATP) |
| 4 mM Mg2+-ATP | SIS + 4.27 mM MgCl2 + 6 mM Na+-ATP | (2 mM free ATP) |
| 6 mM Mg2+-ATP | SIS + 6.27 mM MgCl2 + 9 mM Na+-ATP | (3 mM free ATP) |
| For Figure 6 (MSCs—always 0 mM Mg2+) | ||
| 0 mM Na+-ATP | SIS | (0 mM free ATP) |
| 6 mM Na+-ATP | SIS + 6 mM Na+-ATP | (6 mM free ATP) |