Fig. 4.

ESR spectrum (a) was obtained when aqueous solution containing isolated mitochondria from RGM1 cells with respiratory substrates and 10 mM CYPMPO was incubated for 5 min. ESR spectra (b) and (c) was obtained when aqueous solution containing indomethacin treated isolated mitochondria with (b) or without (c) lansoprazole pretreatment from RGM1 cells with respiratory substrates and 10 mM CYPMPO was incubated for 5 min. ESR spectrum (d) was obtained when aqueous solution containing 250 µM hypoxanthine, 25 mU/ml hypoxanthine oxidase, 0.1 mM DATAPAC and 10 mM CYPMPO was incubated for 5 min. ESR spectrum (e) was obtained when aqueous solution containing indomethacin treated isolated mitochondria from MnSOD-overexpressed RGM1 cells and 10 mM CYPMPO was incubated for 5 min. The ESR spectrum (f) was a computer simulated ESR spectrum. The amount of ROS leakage from the mitochondria without indomethacin-treated cells was significantly lower than that of indomethacin-treated cells. 24 h pretreatment of lansoprazole reduced this indomethacin-induced ROS leakage by half. *p<0.05