Fig. 1.

Liver histology. Mice were treated for 3 days with 10 mg/kg/day of copper and 10 mg/kg/day of PPARβ/δ agonist (GW0742) and/or 1 mg/kg/day of the PPARβ/δ antagonist (GSK0660) and sacrificed at day #4. (A) Control mice were treated with the corresponding vehicles. (B) Modest vacuolization in cells around the central vein and atrophic degeneration was observed in the liver of mice treated with copper. No obvious changes were observed in mice that received GW0742 (C) or copper+GW0742 (D). Mice treated with GSK0660 alone show some vacuolization of cells and changes in nucleus: cytoplasm ratio (E). The mentioned changes were more noticeable in the mice that received copper and GSK0660 (F). Liver of all mice in each group were processed, assessing all fields in one section of each; a representative field of each group was selected for this figure. Hematoxylin and eosin staining.