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. 2001 Dec 1;15(23):3169–3182. doi: 10.1101/gad.929001

Figure 5.

Figure 5

Sas2 and Sas4 interacted with Cac1, but not Cac2 or Cac3. (A) Sas2 and Sas4 coimmunoprecipitated with Cac1. Cell extracts were prepared from AEY1558 or AEY1559 expressing myc–Cac1 (pAE614) or HA–Cac1 (pAE544) and myc–Sas4 (pAE613) or Sas2 (pAE240), as appropriate; precipitated with antibodies as indicated; and analyzed by immunoblotting. (B) Cac2 and Cac3 coimmunoprecipitated Cac1, but not Sas2. Extracts from strain AEY1558 expressing HA–Cac1 (pAE544), myc–Cac2 (pAE820), and Sas2 (pAE240) or strain AEY2463 (Cac3–myc) expressing HA–Cac1 (pAE544) and Sas2 (pAE240) were immunoprecipitated with α-myc and immunoblotted with α-HA (top) or α-Sas2 (bottom).