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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Plant J. 2007 Nov 7;53(3):554–565. doi: 10.1111/j.1365-313X.2007.03364.x

Figure 1. Stability of SOS1 mRNA is regulated by abiotic stresses.

Figure 1

(a) Northern blot detecting the transcripts of the GUS gene under the control of the 35S promoter. Three independent transgenic lines were used. C, control; Na, 150 mm NaCl treatment for 5 h; H2O2, 10 mm H2O2 treatment for 1 h.

(b) Northern blot detecting the transcripts of the GUS gene under the control of the SOS1 promoter. Two independent transgenic lines were used. C, control; Na, 150 mm NaCl treatment for 5 h; H2O2, 10 mm H2O2 treatment for 1 h.

(c) Induction of stability of SOS1 mRNA by NaCl at different concentrations in 35S:SOS1 transgenic plants.

(d) Time course of induction of stability of SOS1 mRNA by NaCl in 35S:SOS1 transgenic plants.

(e) Northern blotting of SOS1 transcripts in wild-type and 35S:SOS1 transgenic plants after different treatments. Salt treatments were done by treating the seedlings with 150 mm salt in 1/2 MS liquid medium for 5 h. Sorbitol: 300 mm sorbitol for 5 h; Cold, 0°C for 24 h; ABA, 100 µm ABA for 3 h; Dehyd, dehydration by drying seedlings on a filter paper for 15 min. Tubulin and rRNA were used as loading controls.