(a) Pharmaceutical study. The SOS1 transcripts were detected in the 35S:SOS1 transgenic plants. The treatments are: 1, Control; 2, 150 mm NaCl for 1 h; 3, 100 µm diphenylene iodonium (DPI) for 3 h; 4, 100 µm DPI for 2 h followed by 150 mm NaCl with DPI for 1 h; 5, 15 mm DMTU for 3 h; 6, 15 mm DMTU for 2 h followed by 150 mm NaCl with DMTU for 1 h; 7, 1 mm DF for 3 h; 8, 1 mm DF for 2 h followed by 150 mm NaCl with DF for 1 h.
(b) Accumulation of native SOS1 transcripts in response to NaCl treatment in wild type and mutants defective in NADPH oxidases. C, control; Na, 200 mm NaCl for 5 h.