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. 2001 Dec 1;15(23):3104–3117. doi: 10.1101/gad.926401

Figure 6.

Figure 6

E4orf6/E1B55K require NEDDylated Cullins to degrade p53. (A) Wild-type and ts41 Chinese hamster ovary (CHO) cells maintained at 33°C were infected with various combinations of adenovirus vectors for 48 h (see + at top). Vectors included AdrtTA (vector control), Adp53wt, AdE4orf6, and AdHH55K. Some cultures were shifted to the 39°C for the last 15 h of incubation. Whole cell extracts analyzed by Western blotting using to detect p53 (1801), E4orf6 (1807), E1B55K (2A6), and actin. (B) CHO cells infected with various combinations of adenovirus vectors (as in A). Proteasome inhibitors MG132, lactacystin, or their solvents were added for the last 6 h of infection. Cell extracts were analyzed as in A.