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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Anal Bioanal Chem. 2010 Sep 14;399(2):559–570. doi: 10.1007/s00216-010-4166-x

Figure 2.

Figure 2

Schematic outline of experiments designed to determine the specificity and activity of heparan sulfate biosynthetic enzymes. A) The scheme illustrates the influence of PAPS concentration on the action of N-deacetylase-N-sulfotransferase (NDST); B) The scheme illustrates the selective incorporation of tritium (3H) at C5-carbon of IdoA generated upon epimerization of GlcA catalyzed by C5-epimerase. Reverse epimerization leads to incorporation of 3H into GlcA residues as well; C) the scheme outlines the incorporation of radioactive sulfates at C6-carbon of GlcNS residues catalyzed by various 6-OST isoforms.