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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Anal Bioanal Chem. 2010 Sep 14;399(2):559–570. doi: 10.1007/s00216-010-4166-x

Figure 4.

Figure 4

Total ion chromatogram of pentasaccharide I (peak 1) and pentasaccharide V (peak 2), having the same number of sulfate groups, resolved based on the sulfation pattern using IPRP-capillary HPLC and subsequently characterized by online micro ESI-TOF-MS. Electrospray mass spectra of pentasaccharides I and V are shown in the lower panel. Pentasaccharide I carries a 6-O-34SO3− group as a mass spectral probe to be distinguished from its isomer pentasaccharide V (see scheme 1 for structural information).