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. 2001 Dec 15;15(24):3286–3295. doi: 10.1101/gad.940201

Figure 1.

Figure 1

H3 lysine 4 (Lys4) methyl-specific antiserum recognizes H3 Lys4 methylation on peptides and histones. (A) Amino acid sequences of the different H3 peptides used in the ELISA analyses are shown in B. Underlined amino acids are artificial to the H3 sequence and used for coupling purposes. (B) enzyme-linked immunosorbent assay (ELISA) analyses of the α-H3(Lys4)Me antiserum for its recognition of and specificity toward unmodified (unmod[1–8]) and modified H3 (K4Me[1–8]) peptides. Peptide competitions were performed with 20 μg/mL H3 peptides K4Me(1–20) or K9Me(1–20). (C) Whole cell extracts were isolated from wild-type (Wt) and H3 K4R, K4A, and K9R yeast strains and immunoblotted with α-Me(lys4)H3 or α-acetyl H4 and α-H3 antiserum as a loading controls. Wt, K4R, K4A, and K9R were introduced into the MSY421 background.