FIG. 1.

Concavalin A (Con A) lectin capture of serum glycoproteins. A nondisease human pooled serum sample (20 μL) was processed under different conditions, and the resulting proteins separated on an 8–16% SDS-gel, visualized by Coomassie blue staining. Lane 1, unprocessed serum, 1 μL loaded; lane 2, serum depleted of albumin and immunoglobulins using a ProteoPrep AI column from Sigma Chemicals; lane 3, serum depleted of abundant proteins using a Montage column from Millipore; lane 4, serum incubated with agarose bound Con A lectin. Bound glycoproteins were eluted with 0.2 mM alpha-methyl mannoside; lane 5, ProteoPrep AI depleted serum proteins incubated with Con A lectin, followed by elution; lane 6, montage-depleted serum proteins incubated with Con A lectin, followed by elution.