FIG. 2.

Staged breast cancer pooled sera with fucose lectin affinity capture (AAL) and (AAA). Pooled sera (20 μL) from each condition was incubated with the indicated agarose-bound lectin, aleuria aurantia (AAL), or anguilla anguilla (AAA), for 16 h. Bound glycoproteins were eluted with 0.2 mM L-Fucose, separated on an 8–16% SDS-gel and silver stained.