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. 2011 May;15(5):251–259. doi: 10.1089/omi.2010.0120

FIG. 3.

FIG. 3.

MALDI-TOF mass spectrometric analysis of human breast tissue with areas of invasive ductal carcinoma. A 10-μm slice of Sakura/UMFix preserved breast tissue was processed for MALDI imaging and coated with SPA matrix followed by spectra acqusition in linear mode on a Bruker UltraFlexIII MALDI-TOF/TOF mass spectrometer. Spectra were normalized and individual m/z values visualized using FlexAnalysis and FlexImaging software, as previously described (Cazares et al., 2009). (A) Representative histology image of H&E stained breast tissue showing pathologist determined areas of invasive ductal carcinoma in the highlighted ovals. (B) Colorimetric intensity of expression levels of a peptide at m/z 4,588. Areas of most intense expression are in red (see color bar for scale). (C) Differential color intensity comparison of two peaks at 4,588 m/z (red) in the invasive ductal tissue sections relative to a noncancer associated peak at 4,990 m/z (blue).