FIGURE 3:

Reexpression of Oct1 in oct1Δ yeast cells. (A) Radiolabeled Atp2 precursor was incubated with mitochondria from WT^Rho+, oct1Δ, or WT^Rho0 cells over increasing periods of time (3, 6, 12 min). Samples were treated with proteinase K and analyzed by SDS–PAGE and digital autoradiography. For the longest time point, the membrane potential (Δψ) was dissipated prior to the import reaction. p, precursor; m, mature. (B) Mitochondria isolated from oct1Δ yeast cells transformed with the empty control plasmid and oct1Δ OCT1 yeast reexpressing Oct1 from its endogenous promoter were incubated with ³⁵S-Atp2 or ³⁵S-Cox4. Samples were analyzed as described in (A) and Materials and Methods. i, intermediate.