FIGURE 4:

The half-life of Oct1 substrate proteins is prolonged upon full processing of the intermediate forms in organello. (A) Mitochondria from oct1Δ and oct1Δ OCT1 (reexpressing the OCT1 gene) yeast cells were incubated at 37°C for the indicated time. Mitochondria were reisolated and analyzed by SDS–PAGE and immunoblotting. N-terminal amino acids of Oct1 intermediate (Int.) and mature (Mat.) forms of the proteins tested are indicated. (B) Quantification of protein turnover: graphs indicate averaged protein levels of the last incubation time points (166, 76 h) in comparison to the level at 0 h. Values of oct1Δ OCT1 mitochondria were set to 100% (control). Error bars represent SD from three different experiments.