FIGURE 5:

Constitutive activity of Scant. Recombinant Drosophila Gwl proteins, either WT or K97M/Scant (equivalent to K71M Xenopus Gwl), were purified from non-OA–treated Sf9 cells and assayed for activity against a fragment of endosulfine fused to maltose-binding protein as described in Materials and Methods. The first and third rows show Coomassie staining of Gwl and endosulfine proteins used in the assay. The amount of Gwl proteins assayed varied from 1 to 50 ng. The autoradiographs in the second and fourth rows show, respectively, autophosphorylation of Gwl and phosphorylation of the endosulfine fragment fused to maltose-binding protein.