FIGURE 1:

The toxic effects of Dma1/Dma2 lack in cells lacking Hsl1 and Mih1 are counteracted by SWE1 deletion. (A, C) Serial dilutions of stationary-phase cultures of strains with the indicated genotypes were spotted on plates containing either synthetic medium without (−Met) or with (+Met) 2 mM methionine or YEPD (C), which were then incubated for 2 d at 25°C. (B, D) The morphology at 25°C of cells with the indicated genotypes was examined by differential interference contrast (DIC) microscopy. All cell cultures were exponentially growing in YEPD medium, with the exception of those carrying the MET3-HSL1 construct integrated at the HSL1 locus (B), the cellular morphology of which was analyzed after MET3 promoter shutoff for 3 h by 2 mM methionine addition to synthetic growth medium lacking methionine. Bar, 5 μm.