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. 2011 Jul 1;22(13):2185–2197. doi: 10.1091/mbc.E11-02-0127

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© 2011 Raspelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — The lack of Dma proteins causes Swe1 stabilization. Exponentially growing (cyc) cultures of SWE1-3HA (ySP3427) and dma1Δ dma2Δ SWE1-3HA (yRF661) cells were arrested in G1 by α-factor and released from G1 arrest in YEPD at 25°C (time 0), followed by α-factor readdition after 85 min. At the indicated times after release, cell samples were taken for FACS analysis of DNA contents (A), for scoring budding, nuclear division, septin ring formation, and Swe1-3HA localization (B), and for determining Swe1 levels by Western blot analysis with anti-HA and anti-Swi6 (loading control) antibodies (C).