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. 2011 Jul 1;22(13):2185–2197. doi: 10.1091/mbc.E11-02-0127

FIGURE 4:

FIGURE 4:

The lack of Dma proteins causes Swe1 retention at the bud neck in HU-treated cells. Exponentially growing cell cultures of HSL1-18MYC (ySP3157) and dma1Δ dma2Δ HSL1-18MYC (yRF672) strains (A) or SWE1-3HA (ySP3427) and dma1Δ dma2Δ SWE1-3HA (yRF661) strains (B) were transferred to YEPD medium containing 200 mM HU at 25°C. After 3 h, nuclei and septin ring deposition were analyzed as in Figure 3, whereas Hsl1-18MYC and Swe1-3HA were visualized by indirect immunofluorescence with anti-Myc and anti-HA antibody, respectively. Bar, 5 μm.