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. 2011 Jul 1;22(13):2185–2197. doi: 10.1091/mbc.E11-02-0127

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© 2011 Raspelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Septin mutants do not accumulate hyperphosphorylated Swe1 in the presence of HU. Exponentially growing (cyc) cultures of SWE1-3HA (ySP3427), dma1Δ dma2Δ SWE1-3HA (yRF661), cdc12-1 SWE1-3HA (yRF816) and cdc12-6 SWE1-3HA (yRF828) cells were arrested in G1 by α-factor at 23°C and released in YEPD medium containing 200 mM HU. Samples were taken at the indicated times to determine the kinetics of septin ring deposition (A), as well as Swe1 levels (B) by Western blot analysis with anti-HA (Swe1–3HA) and anti-Pgk1 (loading control) antibodies, and for in situ immunofluorescence analysis (C) of nuclei, septin ring, and Swe1-3HA as in Figure 4. Representative micrographs were taken at t = 180 min. Bar, 5 μm.