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. 2011 Jul 1;22(13):2185–2197. doi: 10.1091/mbc.E11-02-0127

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© 2011 Raspelli et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Septin ring stabilization in dma1Δ dma2Δ cells does not restore Swe1 degradation in the presence of HU. Exponentially growing (cyc) cultures of dma1Δ dma2Δ SWE1-3HA cells containing the 2μ plasmid YEplac181, either empty (YEp) (yRF714) or carrying the BNI5 gene (YEp-BNI5) (yRF710), were grown at 25°C in synthetic medium lacking leucine, arrested in G1 by α-factor, and released from G1 arrest in YEPD medium containing 200 mM HU (time 0). Samples were taken at the indicated times after release for determining the kinetics of budding (A) and septin ring deposition (B) and for analysis of Swe1 levels (C) as in Figure 2C.