FIGURE 5:

Deletion of Opa1 causes impaired insulin secretion in mice and in isolated islets. (A) Insulin contents in isolated islets were determined and normalized to total protein amounts (n = 3). (B) Insulin secretion from isolated islets. Isolated islets were incubated with 3 and 11 mM glucose for 1 h. Secreted insulin levels were determined and normalized to total insulin contents (n = 3). (C) Isolated islets were stained with 2 μM Fura 2-AM and stimulated with 3 and 11 mM glucose (n > 4). The 340 nm/380 nm fluorescence ratio was measured under a fluorescence microscope at 8 min after stimulation.