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. 2011 Jul 1;22(13):2290–2300. doi: 10.1091/mbc.E10-11-0893

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© 2011 Kageyama et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Localization of Atg proteins in Atg9L1 KO cells. Atg9L1 KO MEFs and the parental wild-type cells stably expressing GFP-Atg5 (A), GFP-Atg14L (B), GFP-WIPI-1 (C), or ULK1-GFP (D) were infected with S. typhimurium expressing mCherry for 1 h. After fixation, images were taken. The number of Atg-positive bacteria for each condition was determined and the percentages per all bacteria are shown. The average ± SD is shown for three independent experiments where at least 100 bacteria were counted. Scale bar: 20 μm.