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A 185-bp amplicon of TP53 exon 7 was analyzed via HRM after conventional PCR (A) and COLD-PCR (C). Amplicons were subsequently sequenced (Sanger, sense strand). While a 5% mutant abundance of the HCC2157 cell line (C>T) is unreliable in the chromatogram of conventional PCR amplicons (B), a 3% mutant abundance is easily visible in the chromatogram of COLD-PCR (D). Wild-type (WT) sequences are presented for comparison.
