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. 2011 Jul 1;6(7):e21232. doi: 10.1371/journal.pone.0021232

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Luzardo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Ang II receptors (AT₁ and AT₂) were immunodetected in proximal tubule cell membranes of control (C) and programmed (P) offspring. (A) AT₁ receptors. (B) AT₂ receptors. In (A) and (B): upper panels show representative immunoblots after immunoprecipitation with the corresponding antibodies (IP, immunoprecitate; S, supernatant); middle panels show immunodetection of β-actin probed in the same membrane, which was used to asses protein loading in the gels; lower panels show densitometric quantification (means ± S.E.M.) of five immunodetections carried out with different membrane preparations. The band intensity of the respective control group was taken as 100%. *Statistically different from the control group (P<0.05).