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Effect of immobilization on submandibular gland and plasma EGF and on liver c-Fos protein. Animals were immobilized for the indicated period (0, 20, or 180 minutes). Mice were killed, and samples were obtained to determine EGF in submandibular salivary glands and plasma and to quantify relative mass of c-Fos protein in liver homogenates. Each bar corresponds to the mean ± SE of 9–13 animals. Statistical comparisons vs zero time were made by Student's t-test. * P < 0.05; ** P < 0.01
