Skip to main content
. 2011 Jul 1;6(7):e21650. doi: 10.1371/journal.pone.0021650

Figure 1. Prostate epithelial cells immortalization.

Figure 1

To induce immortalization, cells were introduced with the telomerase catalytic subunit hTERT, and both the p53 and pRB pathways were perturbed using p53 knockdown and over-expression of cyclinD/CDK4, respectively. (B) Primary PrECs, as well as hTERT/shp53/CyclinD-CDK4 – overexpressing cells (EP cells), were sequentially passaged and counted. Population Doublings (PDLs) were calculated using the formula: PDLs = log(cell output/cellinput)/log2. (C) EP cells were introduced with AR and either TMPRSS2/ERG (EP-AR TMPRSS2/ERG) or an empty vector (EP-AR). AR and ERG protein levels were measured by Western blot. Actin was used as a loading control.