Figure 6. NafA regulates bacterial aggregation.

The wild-type strain, the ΔNafA strain, or the ΔNafA/NafA strain was suspended in GC-liquid (A), DMEM with 1% FCS (B), and DMEM without FCS (C). The formation of bacterial aggregates was analyzed at indicated time points using a living-cell microscope at 37°C in an atmosphere of 5% CO₂. Arrows in panel A and B indicate small bacterial aggregates. The arrow and arrowhead in panel C show a loose and a spherical bacterial aggregate, respectively.