Table 1. Primers used for cloning.

Name	Sequence of primer pairs
B4-NMC0982	5′-ATAGAAAAGTTGGCAAGGCGAGGTAACGCCGTACTGG-3′
B1R-NMC0982	5′-TGTACAAACTTGTTAAATGCCGTCTGAACATTTTTCAGAC-3′
B2R-NMC0982	5′-TGTACAAAGTGGATGGAAATCAACCCCGAATTGCAGGCTTACG-3′
B3-NMC0982	5′-ATAATAAAGTTGTTTGCCTTGCAGGATGTCGGCAAGG-3′
B1-KmR	5′-AAAAAGCAGGCTGTATTAGTGACCTGTAGAATTCGAGC-3′
B2-KmR	5′-AGAAAGCTGGGTTTAGAAAAACTCATCGAGCATCAAATG-3′
B4-DUS-5	5′-ATAGAAAAGTTGATGCCGTCTGAAAATTAAGTTAGAATTATCCC-3′
5-porA-pro-L	5′-CGCGGCACTTTAAAATCCAAAATCATACTGCC-3′
5-porA-pro-U	5′-GATTTTGGATTTTAAAGTGCCGCGTGTGTTTTTTTATGGC-3′
B1R-porA-pro	5′-TGTACAAACTTGATCTGCTTCCTTTTGTAAATTTGATAAAAACC-3′
B2R-Cm-U	5′-TGTACAAAGTGGCTAAGTTGGCAGCATCACCC-3′
Cm-3-L	5′-TGGTATCCGACCATATTGACATCATATATGCC-3′
Cm-3-U	5′-TGATGTCAATATGGTCGGATACCAATCTGACG-3′
B3-3	5′-ATAATAAAGTTGCTAACAGAAAACTCTACTCC-3′
B1-NMC0982-comp	5′-AAAAAGCAGGCTTCGGCGCAAAGGGCAACAGCC-3′
B2-NMC0982-comp	5′-AGAAAGCTGGGTTCAGGCGTAAGCCTGCAATTCGGGG-3′
pilE-U	5′-TATTCCGACAACGGCACATTCCC-3′
pilE-L	5′- CCTTCAACCTTAACCGATGCCA-3′
rpsJ-U	5′-TTGGAAATCCGCACCCACTT-3′
rpsJ-L	5′-TACATCAACACCGGCCGACAAA-3′
rplP-U	5′-GTGGCGGTAAAGGTAACGTGGAAT-3′
rplP-L	5′-TCGAATGCTTCACGAGCCAGTT-3′

^*The DNA uptake sequence is marked in italic.

The overlapping homologous sequence for combined PCR is underlined.