Fig 5.

Influence of T gondii HSP70 on NO release. Age/sex-matched WT B6 (A) and IKO B6 (B) were i.p. infected with 200 cysts of T gondii. At 3 h P.I., PECs from mice were harvested and highly purified peritoneal macrophages were obtained by adhering to plastic plates. For further handling, the peritoneal macrophages were cultured at a concentration of 2 × 10⁵ cells per well in a 96-well microtiter plates. Recombinant mIFN-γ and rT gondii HSP70 were added simultaneously with peritoneal macrophages and were present in culture medium for the entire cultivation time of 12 h. NO₂⁻ was measured 12 h post treatment by the Griess reaction. The levels of NO release of non-stimulated peritoneal macrophages from non-infected WT and IKO B6 mice were 2.2 and 0.8, respectively. *,P<0.05; **, P <0.005. Three mice were used for each experimental group and the experiment was repeated at least 2 times