Skip to main content
. 2011 Jul 1;6(7):e21860. doi: 10.1371/journal.pone.0021860

Figure 1. Overview of the three SSH libraries.

Figure 1

(A) Schematic of SSH library construction. The D–T library was constructed using D-shape stage larval cDNA as the tester and trochophore stage larval cDNA as the driver. The U–D library was constructed using umbonal stage larval cDNA as the tester and D-shape stage larval cDNA as the driver. The J–U library was constructed using juvenile stage larval cDNA as the tester and umbonal stage larval cDNA as the driver. Bar  = 15 µm in oosperm, 15 µm in trochophore and D-shaped stages, 40 µm in umbonal and juvenile stages, and 4 cm in the adult stage. (B) Length distribution of unigenes in the three SSH libraries. The white columns represent unigenes in the D–T library, grey columns represent unigenes in the U–D library, and the dark grey columns represent unigenes in the J–U library. Unigene-length frequencies for each library are plotted in 100-bp bins.