Figure 2. Secreted proteins predicted in the three SSH libraries.
Unigenes with a coding region >150 base pairs, processing a signal sequence, predicted to be secreted proteins using TargetP [88] on TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/), and GPI modification predictor [89] together [90]. The unigenes coding for secreted proteins were searched by BLASTX using the Genbank nr database with a cutoff e-value of 10e–05. (A) Unigenes in the D–T library. (B) Unigenes in the U–D library. (C) Unigenes in the J–U library.