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. 2011 Mar;13(2):143–151. doi: 10.1016/j.jmoldx.2010.10.005

Figure 2.

Figure 2

Effect of archival age on housekeeping gene expression in FFPE diabetes biopsy specimens. Expression of GAPDH and 18s housekeeping genes in 120 archived FFPE biopsy specimens were measured in triplicate using conventional real-time PCR. Mean amplification cycle thresholds (Ct) and SD are shown according to age of biopsy (A; archive time). For 8 years of archival time, the expression of GAPDH was relatively constant, and there was modest variability in expression levels. In contrast, there was a significant time-dependent decrease in 18s expression, and there was high variability in 18s expression in more recent biopsy specimens. B shows the time-dependent variability in 18s expression.