Fig 5.

Immunoblot analysis of human CCT, mitochondrial Hsp60, and E coli GroEL using human sera. CCT, Hsp60, or GroEL were resolved on native PAGE or SDS-PAGE gels, blotted onto membranes, and incubated with human sera (diluted 100-fold) followed by alkaline phosphatase–conjugated anti-human IgG. Healthy control serum samples no. 52 and 72 were positive (+) for antibodies against all the Hsp60 family proteins by ELISA, whereas no. 63 and 67 were negative (−). The patients' sera selected for this assay showed high antibody titers against all the antigen tested by ELISA. Arrowheads indicate the position of each antigen. Mobility of molecular weight markers are indicated at the left