. Author manuscript; available in PMC: 2012 Jul 4.

Published in final edited form as: Angew Chem Int Ed Engl. 2011 Jun 7;50(28):6258–6263. doi: 10.1002/anie.201102459

Table 1.

Comparison of chemical and optical properties of variously charged NIR fluorophores in 100% FBS.

Property	CW800	RS800	ICG	ZW800-1	ZW800-3a
MW (Da)	1091	887	775	1149	945
Net Charge	-4	-2	-1	0	+2
LogD at pH 7.4	2.51	0.11	7.88	-3.56	-6.95
ε (M^-1cm^-1)	237,000	240,000	121,000	249,000	309,000
λ_exc (nm)	786	784	807	772	774
λ_em (nm)	800	800	822	788	790
Stokes Shift (nm)	14	16	15	16	16
Φ (%)	14.2	16.9	9.3	15.1	16.1
Decomposition (%)	4.0	4.4	5.9	5.5	3.8

CW800 = IRDye™800-CW carboxylic acid; ICG = indocyanine green; RS800 = IRDye™800-RS carboxylic acid; ZW = zwitterionic.