Figure 2.

(A) A plot of the mean levels of circulating blood leukocytes (overall white blood cells [WBC], lymphocytes, granulocytes, and monocytes) after combined bone marrow and kidney transplantation shows that a nadir of the circulating cells occurs at 7–10 days, just at the onset the engraftment syndrome. Granulocytes and monocytes recover by day 21, a time when the kidney function is also improving. Plotted are means from patients #2, 4, 6, 7, 8, 9, and 10 and the standard error of the mean. (B) Flow cytometry shows that the mean % donor circulating CD3⁺CD4⁺ and CD3⁺CD8⁺ cells vary in the post-transplantation period, peaking around day 7. Data is given as an average of patients 1–7 and 9–10, with box plots depicting the distribution of the data. Individual data points on each day are depicted. (C) For patients with available data, flow cytometry shows a transient upregulation of CD25⁺ among CD8⁺ T cells during the peri-transplant period. The percentage of CD25⁺CD8⁺ cells in gated CD3⁺CD8⁺ cells was determined in all patients on days 7, 10 and 14 post-transplant. Additional determinations were available pre-transplant (day -7) in patients 7 to 10, and at day 0 and 2 post-transplant in patients 6 and 7.